Effects of strand and directional asymmetry on base-base coupling and charge transfer in double-helical DNA

Mechanistic models of charge transfer (CT) in macromolecules often focus on CT energetics and distance as the chief parameters governing CT rates and efficiencies. However, in DNA, features unique to the DNA molecule, in particular, the structure and dynamics of the DNA base stack, also have a dramatic impact on CT. Here we probe the influence of subtle structural variations on base-base CT within a DNA duplex by examining photoinduced quenching of 2-aminopurine (Ap) as a result of hole transfer (HT) to guanine (G). Photoexcited Ap is used as a dual reporter of variations in base stacking and CT efficiency. Significantly, the unique features of DNA, including the strandedness and directional asymmetry of the double helix, play a defining role in CT efficiency. For an (AT)(n) bridge, the orientation of the base pairs is critical; the yield of intrastrand HT is markedly higher through (A)n compared with (T)(n) bridges, whereas HT via intrastrand pathways is more efficient than through interstrand pathways. Remarkably, for reactions through the same DNA bridge, over the same distance, and with the same driving force, HT from photoexcited Ap to G in the 5' to 3' direction is more efficient and less dependent on distance than HT from 3' to 5'. We attribute these differences in HT efficiency to variations in base-base coupling within the DNA assemblies. Thus base-base coupling is a critical parameter in DNA CT and strongly depends on subtle structural nuances of duplex DNA

Mapping, sensing and visualising the digital co-presence in the public arena

This paper reports on work carried out within the Cityware project using mobile technologies to map, visualise and project the digital co-presence in the city. This paper focuses on two pilot studies exploring the Bluetooth landscape in the city of Bath. Here we apply adapted and ‘digitally augmented’ methods for spatial observation and analysis based on established methods used extensively in the space syntax approach to urban design. We map the physical and digital flows at a macro level and observe static space use at the micro level. In addition we look at social and mobile behaviour from an individual’s point of view. We apply a method based on intervention through ‘Sensing and projecting’ Bluetooth names and digital identity in the public arena. We present early findings in terms of patterns of Bluetooth flow and presence, and outline initial observations about how people’s reaction towards the projection of their Bluetooth names practices in public. In particular we note the importance of constructing socially meaningful relations between people mediated by these technologies. We discuss initial results and outline issues raised in detail before finally describing ongoing work

Farm Technical Efficiency and Extension

This paper presents a methodology for estimating technical efficiency levels for individual farms using both a fixed effects panel model and a stochastic production frontier approach. It tests whether the estimated technical efficiency levels are associated with measures of contact with the advisory service. The approach is applied to a panel of 307 farms drawn from the Irish National Farm Survey over the period 1984 to 1994. The results show evidence that extension contact has had a positive impact on agricultural output.

Statistical Mechanics of 2+1 Gravity From Riemann Zeta Function and Alexander Polynomial:Exact Results

In the recent publication (Journal of Geometry and Physics,33(2000)23-102) we demonstrated that dynamics of 2+1 gravity can be described in terms of train tracks. Train tracks were introduced by Thurston in connection with description of dynamics of surface automorphisms. In this work we provide an example of utilization of general formalism developed earlier. The complete exact solution of the model problem describing equilibrium dynamics of train tracks on the punctured torus is obtained. Being guided by similarities between the dynamics of 2d liquid crystals and 2+1 gravity the partition function for gravity is mapped into that for the Farey spin chain. The Farey spin chain partition function, fortunately, is known exactly and has been thoroughly investigated recently. Accordingly, the transition between the pseudo-Anosov and the periodic dynamic regime (in Thurston's terminology) in the case of gravity is being reinterpreted in terms of phase transitions in the Farey spin chain whose partition function is just a ratio of two Riemann zeta functions. The mapping into the spin chain is facilitated by recognition of a special role of the Alexander polynomial for knots/links in study of dynamics of self homeomorphisms of surfaces. At the end of paper, using some facts from the theory of arithmetic hyperbolic 3-manifolds (initiated by Bianchi in 1892), we develop systematic extension of the obtained results to noncompact Riemannian surfaces of higher genus. Some of the obtained results are also useful for 3+1 gravity. In particular, using the theorem of Margulis, we provide new reasons for the black hole existence in the Universe: black holes make our Universe arithmetic. That is the discrete Lie groups of motion are arithmetic.Comment: 69 pages,11 figures. Journal of Geometry and Physics (in press

Theology at the coal-face of hospitalisation - the development and evaluation of a postgraduate certificate in Healthcare Chaplaincy

Healthcare chaplaincy is working towards recognition as a registered health profession. An accredited programme of professional education is part of that process. The University of Glasgow supported by NHS Education for Scotland have developed a programme of professional education for healthcare chaplains that is integrated into an MSc. (MedSci) in healthcare. This article outlines the commissioning, development and evaluation of a postgraduate certificate in healthcare chaplaincy by students, clinical mentors and experienced healthcare chaplains. It also highlights an innovative approach to practice development in spiritual and religious care in healthcare

Superorbital Period in the High Mass X-ray Binary 2S 0114+650

We report the detection of a superorbital period in the high-mass X-ray binary 2S 0114+650. Analyses of data from the Rossi X-ray Timing Explorer (RXTE) All-Sky Monitor (ASM) from 1996 January 5 to 2004 August 25 reveal a superorbital period of 30.7 +/- 0.1 d, in addition to confirming the previously reported neutron star spin period of 2.7 h and the binary orbital period of 11.6 d. It is unclear if the superorbital period can be ascribed to the precession of a warped accretion disc in the system.Comment: 5 pages, 5 figures, submitted to MNRAS 27th January 2005. Manuscript expanded to include discussion of evolution of periods, and hardness ratio variability. Number of figures increased from 5 to 9. Accepted for publication 19th December 200

Towards an expanded model of litigation

Introduction: The call for contributions for this workshop describes the important new challenges for the legal search community this domain brings. Rather than just understanding the challenges this domain poses in terms of their technical properties, we would like to suggest that understanding these challenges as socio-technical challenges will be important. That is, as well as calling for research on a technical level to address these challenges we are also calling for work to understand the social practices of those involved in e-discovery (ED) and related legal work. A particularly interesting feature of this field is that it is likely that search technologies will (at least semi-)automate responsiveness review in the relatively near term and this will change the way that the work is organised and done in many ways – offering new possibilities for new ways of organising the work. As well as designing those technologies for automating responsiveness review we need to be envisioning how the work will be done in the future, how these technologies will impact the organisation of the case and so on. In this position paper we therefore outline the importance of understanding the wider social context of ED when designing tools and technologies to support and change the work. We would like to reinforce and expand on Conrad’s call for IR researchers to understand just what ED entails [2], include the stages that come both before and after core retrieval activities. The importance of considering the social aspects of work in the design of the technology has been established for some time. Ushering in this ‘turn to the social,’ and focusing on interface design, Gentner and Grudin [4] described how the GUI has already changed from an interface for engineers, representing the engineering model of the machine to one that supported single ‘everyman’ users (based on ideas from psychology). From then onwards the interface has evolved to support groups of users, taking into account the social and organisational contexts of use. This has particular resonance for the design of ED technologies: during ED in particular and the wider legal process there are often many lawyers involved – reviewing documents, determining issues, etc. Even if the way that their work is organised currently is not seen as collaborative in the traditional sense – with individual lawyers working on individual document sets to review them - their work needs to be coordinated and it seems likely that their work could be enhanced by, for example, knowledge of what their colleagues had found, how the case was shaping up, new key terms and facts turned up and so on. Work is often modelled for the purposes of design using process models, but this misses out on the richness and variety actually found when one examines how the work is carried out [3]. Technologies which strictly enforce the process models can often hinder the work, or end up being worked around as was the case with workflow systems since people interpret processes very flexibly to get the work done ([1], [3]). Other studies in other fields have found similar problems when systems are designed on for example cognitive models of how the work is done; they often do not take into account the situated nature of the work and thus they can be very difficult to use [5]. We believe, like [2], that a clear understanding of the social practices of ED is vital for the creation of high-quality, meaningful tools and technologies. We furthermore propose that work practice studies, to be used in combination with other methods, are a central part of getting the detailed understanding of the work practices central to designing useful and intelligent tools. Work practice studies would involve ethnographies, consisting primarily of observation, undertaken of practitioners engaging in the work of ED